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| Souza,Patrícia Mendes de; Marinho,Petrusk Homero; Lima,Marcos Antônio Barbosa de; Nascimento,Aline Elesbão do; Takaki,Galba Maria de Campos. |
| The aim of this work was to evaluate the physiological aspects of polyphosphate metabolism of Cunninghamella elegans grown in presence of copper. The growth profile was obtained by means of biomass yields, orthophosphate consumption, polyphosphate accumulation and phosphatases activities. The results revealed the influence of copper on the growth, observed by biomass yields. Orthophosphate consumption was faster in cells grown in the presence of copper. The presence of copper in the culture medium induced polyphosphate accumulation. The polyphosphate level was almost constant in the beginning of control culture growth, and could be related to the exponential growth phase. On the other hand, the copper treated cultures exhibited a significant reduction in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cunninghamella elegans; Copper; Polyphosphate; Phosphatases. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400002 |
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| Lima,Marcos Antonio Barbosa de; Nascimento,Aline Elesbão do; Souza,Wanderley de; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de. |
| The content of inorganic polyphosphate and the polymeric degree of these compounds were evaluated during the growth of Cunninghamella elegans in medium containing varying orthophosphate (Pi) concentrations. For this purpose, a combination of chemical methods for polyphosphate extraction and ultrastructural cytochemistry were used. The orthophosphate and glucose consumption was also determined during the fungal cultivation. At Pi concentrations of 0.5, 2.5 and 0.0 g/L, the maximum amounts of biomass were 3.18, 3.29 and 0.24 g/L, respectively. During growth the cells accumulated Pi from the medium. At three days of growth the biomass consumed up to 100 and 95% of Pi from the media at initial concentrations of 0.5 and 2.5 g/L, respectively. Polyphosphate was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polyphosphate; Accumulation; Ultrastructural cytochemistry; Cunninghamella elegans; Ortophosfate. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400016 |
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| Franco,Luciana de Oliveira; Maia,Rita de Cássia C.; Porto,Ana Lúcia F.; Messias,Arminda Sacconi; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de. |
| Chitin and chitosan were extracted from mycelial biomass of Cunninghamella elegans and the performance for copper, lead and iron biosorption in aqueous solution was evaluated. The growth curve of C. elegans was accomplished by determination of biomass, pH, glucose and nitrogen consumption. Chitin and chitosan were extracted by alkali-acid treatment and the yields were 23.8 and 7.8%, respectively. For the adsorption analysis, the process of heavy uptake metal sorption was evaluated using polysaccharides solutions (1% w/v). The rate of metallic biosorption was dependent upon the concentration and pH of metal solutions, and the best results were observed with pH 4.0. Chitosan showed the highest affinity for copper and chitin for iron adsorption. The results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cunninghamella elegans; Chitin; Chitosan; Bioremediation; Biosorption. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200013 |
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